HEX carboxylic acid, 6-isomer

Cat. # Quantity Price Lead time
2B290 5 mg $125 21 days
4B290 25 mg $225 21 days
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Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.

HEX carboxylic acid is a non-reactive form of HEX dye that can be used as a reference standard in experiments involving HEX dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of HEX, 6-isomer

Absorption and emission spectra of HEX, 6-isomer

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ROX carboxylic acid, 6-isomer

Derivative of ROX (Rhodamine X, Rhodamine 101) fluorophore with non-activated free carboxylic acid group. This reagent is a pure 6-isomer.

General properties

Appearance: orange powder
Molecular weight: 582.99
Molecular formula: C21H6Cl6O7
Solubility: good in DMSO, DMF, methanol, basic solutions, limited in acetonitrile
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 87770
Emission maximum, nm: 549
Fluorescence quantum yield: 0.57
CF260: 0.30
CF280: 0.13
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