sulfo-Cyanine3 hydrazide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11370 | 1 mg | $125 | in stock | |
| 21370 | 5 mg | $325 | in stock | |
| 41370 | 25 mg |
$850
|
in stock | |
| 51370 | 50 mg |
$1490
|
in stock | |
| 61370 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.
Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
Absorption and emission spectra of sulfo-Cyanine3
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Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.General properties
| Appearance: | dark red solid |
| Molecular weight: | 630.78 |
| CAS number: | 2144762-62-7 |
| Molecular formula: | C30H38N4O7S2 |
| IUPAC name: | 3H-Indolium, 2-[3-[1-(6-hydrazinyl-6-oxohexyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1,3,3-trimethyl-5-sulfo-, inner salt |
| Solubility: | soluble in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (90%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
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