TCO phosphoramidite C6
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
4017-100mg | 100 mg | $480 | in stock | |
4017-250mg | 250 mg | $920 | in stock | |
4017-1g | 1 g | please inquire | in stock |
Trans-cyclooctene (TCO) amidite is a hydroxyl reactive block used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group, and is especially useful in preparation of TCO-modified oligonucleotides.
Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term stability in aqueous media, which is important in low-concentration systems applications such as protein-protein conjugations, etc.
Recommendations for using the reagent:
Coupling: Standard conditions identical to normal nucleobases.
Oxidation: TCO well tolerates iodine oxidation step conditions.
Deprotection: Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Cleavage: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65°C
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Appearance: | colorless to yellowish oil |
Molecular weight: | 469.61 |
Molecular formula: | C24H44N3O4P |
Quality control: | NMR 1H, NMR 31P (95+%) |
Storage conditions: | 12 months after receival at -20 °C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Oligo synthesis details
Coupling conditions: | Standard conditions identical to normal nucleobases |
Cleavage conditions: | AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C |
Deprotection conditions: | Dmt-ON protocol |