TCO phosphoramidite C6

Oligonucleotide synthesis Copper-free click chemistry Cycloalkenes Phosphoramidites

Cat. #	Quantity	Price	Lead time
4017-100mg	100 mg	$480	in stock
4017-250mg	250 mg	$920	in stock
4017-1g	1 g	please inquire	in stock

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Trans-cyclooctene (TCO) amidite is a hydroxyl reactive block used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group, and is especially useful in preparation of TCO-modified oligonucleotides.

Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term stability in aqueous media, which is important in low-concentration systems applications such as protein-protein conjugations, etc.

Recommendations for using the reagent:

Coupling: Standard conditions identical to normal nucleobases.

Oxidation: TCO well tolerates iodine oxidation step conditions.

Deprotection: Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Cleavage: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65°C

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General properties

Appearance:	colorless to yellowish oil
Molecular weight:	469.61
Molecular formula:	C₂₄H₄₄N₃O₄P
Quality control:	NMR ¹H, NMR ³¹P (95+%)
Storage conditions:	12 months after receival at -20 °C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Oligo synthesis details

Coupling conditions:	Standard conditions identical to normal nucleobases
Cleavage conditions:	AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C
Deprotection conditions:	Dmt-ON protocol

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.