AF 488 dUTP

Cat. # Quantity Price Lead time
5023-50nmol 50 nmol $205 in stock
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AF 488 dUTP is a nucleotide labeled with the fluorescent dye AF 488, used to synthesize labeled DNA probes. Nucleotides can be incorporated into nucleic acid using standard molecular biology techniques such as nick translation, random primer labeling, reverse transcription, PCR, and end-labeling with terminal deoxynucleotidyl transferase. Labeled DNA probes can be used for techniques such as FISH, microarrays, and blotting.

AF 488 is a fluorescent dye that is insensitive to pH in the range from 4 to 10. AF 488 has absorption maxima at 495 nm and emission maxima at 519 nm, which corresponds to the green region of the spectrum.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3.

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General properties

Appearance: orange solid
Molecular weight: 1177.59
Molecular formula: C39H40Li4N6O25P3S2-
Solubility: water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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