Cyanine3 tetrazine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 110E0 | 1 mg | $125 | in stock | |
| 210E0 | 5 mg | $260 | in stock | |
| 410E0 | 25 mg |
$510
|
in stock | |
| 510E0 | 50 mg |
$895
|
in stock | |
| 610E0 | 100 mg |
$1490
|
in stock |
Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with cyclopropenes and strained non-benzo-fused cycloalkynes.
Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.
Absorption and emission spectra of Cyanine3
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A dye with a dibenzocyclooctyne substituting group for the ROX channel.JOE azide, 5-isomer
JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistryCyanine3 phosphoramidite
Phosphoramidite for the synthesis of 5’-Cyanine3-labeled oligonucleotides.General properties
| Appearance: | dark red solid |
| Mass spec M+ increment: | 611.4 |
| Molecular weight: | 727.64 |
| CAS number: | 2183473-57-4 |
| Molecular formula: | C40H46N7BF4O |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Bai, M.; Cao, X.; Huang, W.; Chen, F.; Zhao, Y.; Xue, J.; Li, Y.; Cheng, Y.; Zhang, L.; Zhao, Y. Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-primed DNA Amplification. Analytical Chemistry, 2020, 92(12), 8444–8449. doi: 10.1021/acs.analchem.0c01161
- Moutsiopoulou, A.; Broyles, D.; Joda, H.; Dikici, E.; Kaur, A.; Kaifer, A.E.; Daunert, S.; Deo, S.K. Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. Analytical Chemistry, 2020, 92(11), 7393–7398. doi: 10.1021/acs.analchem.0c00518
- Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852
Short link - lumiprobe.com/sh/p/3Z
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