AF 594 azide

Azides AF 594 Click chemistry

Cat. #	Quantity	Price	Lead time
S7830	250 ug	–	in stock
A7830	1 mg	$140	in stock
B7830	5 mg	$450	in stock
D7830	25 mg	$1460	in stock
E7830	50 mg	$1890	in stock
F7830	100 mg	$2640	in stock

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AF 594 is a water-soluble red-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to Texas Red in spectral characteristics (absorption max. at 586 nm, emission max. at 613 nm) and is not sensitive to pH changes within the range from 4 to 10.

AF 594 azide is a fluorescently labeled azide that reacts with alkynyl derivatives of biomolecules (terminal alkynes and cyclooctynes) via click reactions to form stable adducts. AF 594 azide is commonly used for bioconjugation tasks and labeling of cells in flow cytometry, fluorescent microscopy, and other applications.

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Absorption and emission spectra of AF 594

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	dark-blue crystals
Molecular weight:	842.98
Molecular formula:	C₃₈H₃₉KN₆O₁₀S₂
Solubility:	good in water, DMF, DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	586
ε, L⋅mol⁻¹⋅cm⁻¹:	105000
Emission maximum, nm:	613
Fluorescence quantum yield:	0.77
CF₂₆₀:	0.28
CF₂₈₀:	0.51

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.