HMRhoNox-M, Fe(II)-selective fluorescent probe

Microscopy Flow cytometry
Cat. # Quantity Price Lead time
3317-50ug 50 ug $120 in stock
3317-500ug 500 ug $590 in stock
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HMRhoNox-M (also known as LysoRhoNox) is a Fe2+-selective fluorescent probe based on the N-oxide-controlled spirocyclization of tetramethyl-hydroxymethyl rhodamine.

In the absence of Fe2+, HMRhoNox-M exists in the non-fluorescent spirocyclic form showing only negligible fluorescence in an aqueous buffer and at physiological pH. The addition of Fe2+ induces a 60-fold increase of the fluorescence signal at 575 nm through the deoxygenation of the dialkylamino group and the transition of the probe to an open fluorescent form. HMRhoNox-M responds to Fe2+ in a dose-dependent manner.

The fluorescence response of HMRhoNox-M is highly selective for Fe2+ over other transition metal ions, including Fe3+, alkali metal ions, and alkaline earth metal ions.

HMRhoNox-M is the cell-permeant probe that is mainly localized in lysosomes. It is suitable for monitoring fluctuations of endogenous labile iron in living cells, including the transferrin-induced Fe uptake.

HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
Structure of HMRhoNox-M
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Product in action

A549 cells stained with Fe(II)-selective fluorescent probe HMRhoNox-M (3317-x) and DAPI

Absorption and emission spectra of HMRhoNox-M

Absorption and emission spectra of HMRhoNox-M

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General properties

Appearance: beige-pinkish crystals
Molecular weight: 388.47
Molecular formula: C24H24N2O3
Solubility: DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
Emission maximum, nm: 575
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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