ROX maleimide, 5-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
11280 | 1 mg | $110 | in stock | |
21280 | 5 mg | $165 | in stock | |
41280 | 25 mg | $285 | in stock | |
51280 | 50 mg |
$495
|
in stock | |
61280 | 100 mg |
$880
|
in stock |
ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.
This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.
Absorption and emission spectra of ROX

Recommended protocol
Calculator
Customers also purchased with this product
TAMRA maleimide, 6-isomer
TAMRA is a well-known xanthene dye of the rhodamine series. This reagent is a thiol-reactive maleimide for the labeling of SH groups in proteins and peptides. Pure 6-isomer.sulfo-Cyanine7 maleimide
Water-soluble near-infrared dye maleimide for the labeling of proteins and peptides.Cyanine3B maleimide
Derivative of a Cyanine3B dye, improved Cyanine3 dye to have higher quantum yield, for maleimide reaction chemistry.General properties
Appearance: | red powder |
Mass spec M+ increment: | 656.3 |
Molecular weight: | 656.73 |
Molecular formula: | C39H36N4O6 |
Solubility: | good in DMSO, DMF |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 570 |
ε, L⋅mol−1⋅cm−1: | 93000 |
Emission maximum, nm: | 591 |
Fluorescence quantum yield: | 1.00 |
CF260: | 0.62 |
CF280: | 0.49 |
Product citations
- Khan, S.; Molloy, J.E.; Puhl, H.; Schulman, H.; Vogel, S.S. Real-time single-molecule imaging of CaMKII-calmodulin interactions. Biophysical Journal, 2024, 123(7), 824-838. doi: 10.1016/j.bpj.2024.02.021
Short link - lumiprobe.com/sh/p/5T
The count of items is incorrect.
