AF 647 maleimide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
16880 | 1 mg | $140 | in stock | |
26880 | 5 mg | $450 | in stock | |
46880 | 25 mg | $1460 | in stock | |
56880 | 50 mg | $1890 | in stock | |
66880 | 100 mg | $2990 | in stock |
AF 647 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 647 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.
AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.
Absorption and emission spectra of AF 647
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Water soluble thiol-reactive derivative of sulfo-Cyanine3, a Cy3 analog.AF 488 hydrazide
AF 488 hydrazide is a carbonyl-reactive dye for conjugating with aldehydes and ketones.General properties
Appearance: | dark blue powder |
Molecular weight: | 1081.40 |
Molecular formula: | C41H47K3N4O15S4 |
Solubility: | good in DMSO, DMF |
Quality control: | NMR 1H and HPLC-MS (90+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 655 |
ε, L⋅mol−1⋅cm−1: | 191800 |
Emission maximum, nm: | 680 |
Fluorescence quantum yield: | 0.15 |
CF260: | 0.09 |
CF280: | 0.08 |