AF 647 maleimide

Cat. # Quantity Price Lead time
16880 1 mg $140 in stock
26880 5 mg $450 in stock
46880 25 mg $1460 in stock
56880 50 mg $1890 in stock
66880 100 mg $2990 in stock
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AF 647 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 647 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.

Absorption and emission spectra of AF 647

Absorption and emission spectra of AF 647

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General properties

Appearance: dark blue powder
Molecular weight: 1081.40
Molecular formula: C41H47K3N4O15S4
Solubility: good in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (90+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08
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