AF 647 maleimide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
16880 | 1 mg | $140 | in stock | |
26880 | 5 mg |
$450
|
in stock | |
46880 | 25 mg |
$1460
|
in stock | |
56880 | 50 mg |
$1890
|
in stock | |
66880 | 100 mg |
$2990
|
in stock |
AF 647 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 647 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.
AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.
Absorption and emission spectra of AF 647

Recommended protocol
Calculator
Customers also purchased with this product
Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.BDP® 630/650 carboxylic acid
BDP 630/650 is a bright fluorophore for far red part of the spectrum. This dye is perfect for microscopy and fluorescence polarization assays. This is a carboxylic acid that can be activated for the coupling, or used as a control.get free express delivery
ROX maleimide, 5-isomer
ROX maleimide is a thiol reactive dye derivative based on isomerically bright rhodamine X fluorophore.General properties
Appearance: | dark blue powder |
Molecular weight: | 1081.40 |
Molecular formula: | C41H47K3N4O15S4 |
Solubility: | good in DMSO, DMF |
Quality control: | NMR 1H and HPLC-MS (90+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 655 |
ε, L⋅mol−1⋅cm−1: | 191800 |
Emission maximum, nm: | 680 |
Fluorescence quantum yield: | 0.15 |
CF260: | 0.09 |
CF280: | 0.08 |
