sulfo-Cyanine5-PEG3-biotin
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2449-1mg | 1 mg | $120 | in stock | |
2449-5mg | 5 mg | $290 | in stock | |
2449-50mg | 50 mg | $1270 | in stock |
sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.
Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.
sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.
The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.
Absorption and emission spectra of sulfo-Cyanine5
Customers also purchased with this product
Biocytin hydrochloride
Lysine derivative of biotin for conjugation with avidin or streptavidin, neuroanatomical tracer.Cyanine3.5 NHS ester
Cyanine3.5 dye NHS ester derivative the labeling of amino-groups in biomolecules.get free express delivery
sulfo-Cyanine5 tyramide
A tyramide derivative of sulfo-Cyanine5, a far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
Appearance: | dark blue solid |
Molecular weight: | 1037.36 |
Molecular formula: | C48H65N6KO11S3 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 271000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.28 |
CF260: | 0.04 |
CF280: | 0.04 |