AF 488 tetrazine, 5-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
118E0 | 1 mg | $140 | in stock | |
218E0 | 5 mg |
$450
|
in stock | |
418E0 | 25 mg |
$1460
|
in stock | |
518E0 | 50 mg |
$1890
|
in stock | |
618E0 | 100 mg |
$2640
|
in stock |
AF 488 is a water-soluble green-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to FITC in spectral characteristics (absorption max. at 495 nm, emission max. at 519 nm) and is not sensitive to pH changes within the range from 4 to 10. This reagent is a pure 5-isomer.
This AF 488 derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick, specific and suitable for in vitro cell labeling.
Absorption and emission spectra of AF 488

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General properties
Appearance: | deep orange powder |
Molecular weight: | 920.07 |
Molecular formula: | C43H53N9O10S2 |
Solubility: | good in methanol, DMSO, DMF, and water |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
ε, L⋅mol−1⋅cm−1: | 71800 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
CF260: | 0.16 |
CF280: | 0.10 |
