FAM tetrazine, 5-isomer

Cat. # Quantity Price Lead time
141E0 1 mg $110 in stock
241E0 5 mg $210 in stock
441E0 25 mg $410 in stock
541E0 50 mg $695 in stock
641E0 100 mg $1190 in stock
Found better price? Let us know and we will propose the way forward!

Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

Customers also purchased with this product

DBCO-NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.

Cyanine5.5 carboxylic acid

Cyanine5.5 dye, free acid form, unactivated. The dye can be considered non-reactive for most applications.

FAM tetrazine, 6-isomer

This molecule is a conjugate of bright fluorophore FAM (fluorescein) and tetrazine, a reactive group that reacts with trans-cycloalkenes in a reaction called IEDDA (inverse electron demand Diels-Alder reaction).

General properties

Appearance: yellow solid
Mass spec M+ increment: 529.12
Molecular weight: 559.53
CAS number: 1619222-85-3
Molecular formula: C31H21N5O6
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.