dsGreen for Real-Time PCR, 100×

Cat. # Quantity Price Lead time
A1010 50 uL –   in stock
11010 100 uL $39 in stock
41010 1 mL $235 in stock
71010 5 mL $510 in stock
91010 50 mL $2670 in stock
Found better price? Let us know and we will propose the way forward!

dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain

Excitation and emission spectra of dsDNA complex with dsGreen

Excitation and emission spectra of dsDNA complex with dsGreen

Recommended protocol

Calculator

Customers also purchased with this product

AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.

sulfo-Cyanine5 azide

Water soluble sulfo-Cyanine5 fluorescent dye azide for click chemistry.

Copper(II)-TBTA complex

A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.

General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
  2. Mazunina, E.P.; Gushchin, V.A.; Kleymenov, D.A.; Siniavin, A.E.; Burtseva, E.I.; Shmarov, M.M.; Mukasheva, E.A.; Bykonia, E.N.; Kozlova, S.R.; Evgrafova, E.A.; Zolotar, A.N.; Shidlovskaya, E.V.; Kirillova, E.S.; Krepkaia, A.S.; Usachev, E.V.; Kuznetsova, N.A.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Trivalent mRNA vaccine-candidate against seasonal flu with cross-specific humoral immune response. Frontiers in Immunology, 2024, 15, 1381508. doi: 10.3389/fimmu.2024.1381508
  3. Koryukov, M.A.; Oscorbin, I.P.; Novikova, L.M.; Gordukova, M.A.; Turina, I.E.; Galeeva, E.V.; Kudlay, D.A.; Filipenko, M.L. A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses. International Journal of Molecular Sciences, 2024, 25(13), 7215. doi: 10.3390/ijms25137215
  4. Bykonia, E.N.; Kleymenov, D.A.; Gushchin, V.A.; Siniavin, A.E.; Mazunina, E.P.; Kozlova, S.R.; Zolotar, A.N.; Usachev, E.V.; Kuznetsova, N.A.; Shidlovskaya, E.V.; Pochtovyi, A.A.; Kustova, D.D.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Major Role of S-Glycoprotein in Providing Immunogenicity and Protective Immunity in mRNA Lipid Nanoparticle Vaccines Based on SARS-CoV-2 Structural Proteins. Vaccines, 2024, 12(4), 379. doi: 10.3390/vaccines12040379
Show more (31)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.