endo-BCN CE-phosphoramidite

Oligonucleotide synthesis Cycloalkynes Phosphoramidites Copper-free click chemistry
Cat. # Quantity Price Lead time
3005-100mg 100 mg $390 in stock
3005-250mg 250 mg $850 in stock
3005-1g 1 g $2380 in stock
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Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, bicyclononine in endo-BCN CE-phosphoramidite provides not significantly different rate of cycloaddition compared to its exo-conformer.

BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.

Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).

endo-BCN CE-Phosphoramidite, 3005-1G
endo-BCN CE-Phosphoramidite, 3005-1G
endo-BCN CE-Phosphoramidite, 3005-1G
Structure of endo-BCN CE-Phosphoramidite
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Copper(II)-TBTA complex

A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.

General properties

Appearance: yellowish oil
Mass spec M+ increment: 343.11
Molecular weight: 481.57
CAS number: 1352811-59-6
Molecular formula: C24H40F1N3O5P
Solubility: good in acetonitrile
Quality control: NMR 1H and 31P (95 %)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: Anhydrous Acetonitrile
Deprotection conditions: identical to protected nucleobases
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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