sulfo-Cyanine3-PEG3-biotin
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3079-1mg | 1 mg | $120 | in stock | |
| 3079-5mg | 5 mg | $290 | in stock |
This fluorescent conjugate is useful for streptavidin-based fluorescent assays and visualization of the streptavidin/avidin-labeled biomolecules. Streptavidin, a protein with a high affinity to biotin, has four binding centers for biotin. The high stability of biotin/streptavidin complex gives the opportunity to build different kinds of assays. For example, a target molecule with affinity to the surface can be conjugated with biotin and immobilized on a solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with the biotin-dye conjugate.
The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.
Absorption and emission spectra of sulfo-Cyanine3
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sulfo-Cyanine3 DBCO
sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water-soluble fluorophore sulfo-Cyanine3 for copper-free click chemistry.DBCO-NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.Alkyne maleimide
Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 1011.32 |
| Molecular formula: | C46H63N6KO11S3 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Short link - lumiprobe.com/sh/p/8O
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