sulfo-Cyanine3-PEG3-biotin
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3079-1mg | 1 mg | $120 | in stock | |
| 3079-5mg | 5 mg | $290 | in stock |
This fluorescent conjugate is useful for streptavidin-based fluorescent assays and visualization of the streptavidin/avidin-labeled biomolecules. Streptavidin, a protein with a high affinity to biotin, has four binding centers for biotin. The high stability of biotin/streptavidin complex gives the opportunity to build different kinds of assays. For example, a target molecule with affinity to the surface can be conjugated with biotin and immobilized on a solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with the biotin-dye conjugate.
The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.
Absorption and emission spectra of sulfo-Cyanine3
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sulfo-Cyanine3 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 1011.32 |
| Molecular formula: | C46H63N6KO11S3 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
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